Journal: BMC Plant Biology

Article Title: Identification and characterization of the three homeologues of a new sucrose transporter in hexaploid wheat ( Triticum aestivum L.)

doi: 10.1186/1471-2229-13-181

Figure Lengend Snippet: Alignment of the amino acid sequences of TaSUT2s with other cereal SUT2s. Amino acid sequences of TaSUT2A, TaSUT2B and TaSUT2D were aligned using the DNAMAN program with SUT2s of barley ( Hordeum vulgare ; HvSUT2), rice ( Oryza sativa ; OsSUT2) and maize ( Zea mays ; ZmSUT2). Similarity in amino acids across all the sequences (92.6%) is indicated by stars. The amino acid sequences were also compared with the CONSENSUS sequence derived from the highly conserved region of functional plant SUTs, where 1 = I, L or V; 2 = F, W or Y. The putative vacuolar targeting dileucine motif is shown in a box, and the histidine residue that appears to be located at or conformationally linked to sucrose binding site of SUTs is shown in bold and underlined. The 12 transmembrane helices, coined as distinct characteristic features of all members of the GPH cation symporter family to which all the known plant SUTs belong, are underlined.

Article Snippet: Sequence homology of TaSUT2A, TaSUT2B and TaSUT2D with other cereal SUT genes was analyzed by using DNAMAN ( http://www.lynnon.com/pc/alignm.html ), and their respective coding sequences were identified by using Open Reading Frame (ORF) finder ( http://www.ncbi.nlm.nih.gov/gorf/gorf.html ).

Techniques: Sequencing, Derivative Assay, Functional Assay, Residue, Binding Assay